Contact an Account ManagerDetermination of isoniazid acetylisoniazid acetylhydrazine and diacetylhydrazine in biological fluids by HPLC
Summary
Type: HPLC
Application #: 103360
Catalog#: 720023.46 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4.6x250mm
Detection: UV 220nm
Conditions: eluent: A) 10mM phosphate buffer pH 5.5 - methanol - acetonitrile - dichloromethane (73:17:9:1),
B) 10mM phosphate buffer pH 5.1 - acetonitrile - dichloromethane (84.2:15:0.8),
flow rate 2ml/min
Authors: von Sassen W
Source: J Chromatogr 338 (1985) 113 - 122
Details
Legend: Chromatograms of urine samples A) before and B) after acidic hydrolysis from a rapid (1) and a slow (2) acetylator after intravenous administration of 10mg/kg isoniazid.
1) isoniazid (INH),
2) ace